Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Other
Publication Acceptance Date: 1/5/2006
Publication Date: 1/5/2006
Citation: Li, R.W., Li, C. 2006. Sodium butyrate induced gene expression changes in the madin-darby bovine kidney epithelial cells [abstract]. Gene expression Omnibus. GSE 3970. Interpretive Summary:
Technical Abstract: The microarray is an ideal genomic tool for scientists to study global expression profiles or to analyze the transcriptomes in cattle. Global gene expression profiling of the bovine kidney epithelial cells regulated by sodium butyrate was investigated with high-density oligonucleotide microarrays. The bovine microarray with ~345,000 features (60mer oligonucleotides) representing 45,383 cattle unique sequences was designed and produced with Maskless Array Synthesizer technology. The 45,383 unique sequences include 40,808 Tentative Consensus sequences from the TIGR Bos taurus Gene Index (BTGI, release 11.0) plus 4,575 singletons from pooled mammary gland and gut tissues. We identified 584 significant genes that are differentially regulated greater than 2-fold by butyrate with the False Discovery Rate (FDR) below 20%. This study presented the genes related to multiple signal pathways such as apoptosis, extracellular matrix remodeling and cell cycle control. The profound changes in gene expression elucidate the molecular basis for the pleiotropic effects of butyrate on biological processes. The findings enables better recognition of the full range of beneficial roles butyrate may play during cattle energy metabolism, cell growth and proliferation, and possibly in fighting gastrointestinal pathogens.