Skip to main content
ARS Home » Northeast Area » Geneva, New York » Plant Genetic Resources Unit (PGRU) » Research » Publications at this Location » Publication #191137

Title: COMPUTATIONAL ANALYSIS OF PUTATIVE RESISTANCE GENE ANALOGS IN RASPBERRY

Author
item Baldo, Angela
item SAMUELIAN, SUREN
item WEBER, COURTNEY

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2006
Publication Date: 3/30/2006
Citation: Baldo, A.M., Samuelian, S., Weber, C. 2006. Computational analysis of putative resistance gene analogs in raspberry. Meeting Abstract. 3rd International Rosaceae Genomics Conference. Pg. 65.

Interpretive Summary: Not Applicable

Technical Abstract: Plant R genes are known to confer resistance to a variety of pathogens in a gene-for-gene mode. Seventy-six putative resistance gene analogs (RGAs) containing conserved domains were amplified from red raspberry Rubus idaeus “Latham” using degenerate primers based on RGAs identified in other Rosaceae species. These sequences were clustered using a variety of methods such as Cap3 and Phrap. The consensus sequences of the resulting contigs and singletons were aligned and compared in a phylogenetic context with RGAs identified from other Rosaceae species. This work is part of a larger project to map RGAs in a red raspberry population segregating for resistance to Phytophthora.