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item Maragos, Chris

Submitted to: Mycotoxin Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/8/2005
Publication Date: 6/1/2006
Citation: Maragos, C.M. 2006. Flourescence polarization for mycotoxin determination. Mycotoxin Research. 22(2):96-99.

Interpretive Summary: Fluorescence polarization immunoassay is a technique well suited for the rapid detection of small molecules, such as mycotoxins, with minimal sample preparation. The manuscript summarizes the use of fluorescence polarization immunoassay for the detection of mycotoxins, including the fumonisins, deoxynivalenol, aflatoxins, ochratoxin A, and zearalenone.

Technical Abstract: Immunoassays have become increasingly important for detection of mycotoxins and the increased use of immunoassays has occurred concomitantly with an increase in monitoring programs worldwide. The number and type of immunoassays have continued to expand to include a wide range of antibody-based techniques including the traditional enzyme-linked immunosorbent assays (ELISAs) and immunoaffinity columns (IAC), but also biosensors and lateral flow devices. Because there are a variety of circumstances under which commodities and foods are tested for mycotoxins, a variety of analytical formats exist to fill these needs. While all analysts desire accurate, reproducible, and repeatable analytical solutions, the selection of an analytical protocol almost invariably requires the additional considerations of cost and ease of use. Such considerations have been a driving force in improvements to mycotoxin immunoassays.