Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 11/1/2005
Publication Date: 1/14/2006
Citation: Zhao, S., Qu, L., Nettleton, D., Peng, J., Wang, Y., Lunney, J.K., Geisert, R., Tuggle, C.K. 2006. Identification Of Differentially Expressed Genes In Endometrium RNAs Collected From Early Pregnant And Cycling Yorkshire And Meishan Gilts Using The Affymetrix Porcine Genechip tm. Plant & Animal Genomes XIV Conference [Abstract]. Plant & Animal Genomes XIV Conference, January 14-16, 2006, San Diego, CA. p. 568. Available: http://www.intl-pag.org/14/abstracts/PAG14_P568.html
Technical Abstract: Functional genomics provides a powerful way for discovering the molecular mechanisms involved in gene expression changes during pre-implantation development of porcine embryos, a stage with high embryonic mortality. RNA from endometrial samples from various stages and pregnancy statuses were profiled in quadruplicate by using the Affymetrix porcine GeneChip': Yorkshire gilts (d12 pregnant (12YP) and cycling (12YC); d15 pregnant (15YP) and cycling (15YC); d18 pregnant (18YP); and Meishan pregnant gilts at d12 (12MP) and d18 (18MP). An ANOVA method was used to identify differentially expressed genes in the following comparisons: pregnant versus cycling, across developmental stages, and between breeds. All genes discussed were found to be significant under the criteria of P < 0.0045 and 5% false discovery rate. There were 485 and 449 genes differentially expressed between 12YP and 15YP and between 12YP and 18YP, respectively. There were 1,499 genes that showed differential expression between 12YC and 15YC, and 88 of these also showed differential expression between 12YP and 15YP. A total of 1,249 genes showed differential expression between 12MP and 18MP. Among these, 268 and 254 genes were also differentially expressed between 12YP and 18YP and between 12YC and 15YC, respectively. We also found 1,375 and 320 genes, respectively, between 12YP and 12MP and between 18YP and 18MP, which showed significant differential expression between breeds. The above genes may be interesting for further investigation of the mechanisms controlling implantation and breed phenotype differences in porcine reproduction. Further bioinformatic and confirmation work is underway to interpret the current data.