Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2005
Publication Date: 1/14/2006
Citation: Cheng, H.H., Zhang, H.M., Muir, W.M., Groenen, M.A., Wong, G.K. 2006. Preliminary results on the validation and application of 3,072 chicken SNPs by genotyping 2,576 commercial and experimental birds [abstract]. Plant and Animal Genome XIV. p. 143. Interpretive Summary:
Technical Abstract: Poultry is the third largest agricultural commodity and at 41% and growing, the primary meat consumed in the USA. To meet increasing consumer demand, the poultry industry needs to be more efficient at selecting superior breeding stock especially for traits that are influenced by the environment, expensive to measure, limited to a single sex, or obtained only from carcasses. Developments including the draft chicken genome sequence, the identification of 2.8+ million single nucleotide polymorphisms (SNPs), and the invention of high-throughput instruments that have the ability to rapidly and economically genotype SNPs offer tremendous opportunities to address this concern and more. In the first large-scale public chicken project, 3,072 SNPs that are evenly-spaced throughout the genome were designed for genotyping on the Illumina platform. To evaluate these SNPs, DNA from 2,576 birds were isolated, which includes material from 1,440 elite chickens (36 lines) and numerous characterized experimental lines. Preliminary results will be presented that address: (1) the validation and allele frequency of the SNPs, (2) the generation of a high-density SNP genetic map and its integration with the genome sequence, (3) the feasibility of genome-wide marker-assisted selection (GMAS) in commercial chicken lines, and (4) the determination of what subset of SNPs would allow traceability of poultry meat or live offspring to its pure line parent.