Submitted to: In Vitro Cellular and Developmental Biology
Publication Type: Abstract only
Publication Acceptance Date: 5/2/2005
Publication Date: 6/1/2005
Citation: Hakim, R.S., Blackburn, M.B., Corti, P., Gelman, D.B., Elsin, K., Loeb, M., Lynn, D.E., Smagghe, G. 2005. The assessment of roles for arylphorin in vivo and in vitro. In Vitro Cellular and Developmental Biology 41: 34A. Interpretive Summary:
Technical Abstract: In insects, developmental responses are organ and tissue specific. As part of studying insect midgut cells in primary tissue cultures, both growth and differentiation factors have been identified from the growth media and its additions. Recently, arylphorin, a component of the fat body extract added to our cultures, was analyzed and determined to have a mitogenic effect on the stem cells of the tobacco budworm Heliothis virescens (Blackburn et al., Arch. Insect Biochem. Physiol, 55, 26-32, 2004). To obtain a broader understanding of the role of this protein, arylphorin and chymotrypsinized arylphorin were added to culture media for both primary and continuous cell cultures, and were evaluated for in vivo effects by addition to the diets of several insect species. The results demonstrated a mitogenic role of arylphorin in midgut primary stem cell cultures of several species, and with the enzymatically-treated material, on a fat body cell line. In vivo, arylphorin provided in the diet increased insect growth in almost all species tested.