Submitted to: Annual International Plant & Animal Genome Conference
Publication Type: Abstract Only
Publication Acceptance Date: 1/14/2006
Publication Date: 1/14/2006
Citation: Mariasegaram, H., Chase, C.C., Chaparro, J.X., Olson, T.A., Niedz, R.P. 2006. Interval mapping confirms slick hair gene localization to bovine chromosome 20 [abstract]. Annual International Plant & Animal Genome Conference. January 14-18, 2006, San Diego, CA. p. 237. Interpretive Summary:
Technical Abstract: Results from a DNA pooling study using slick and normal hair coat daughters of a heterozygous Senepol sire mated to Holstein dams pointed to the presence of a slick hair locus on chromosome 20. This was followed by individual genotyping and interval mapping to confirm the presence, and refine the location of the gene along the chromosome. A total of 14 microsatellite markers were individually genotyped across all of the cows constituting the slick and normal pools, as well as in two additional three-generational Senepol families that were both dairy and beef-based. Interval mapping was carried using Superlink, a program that uses Baysean networks to calculate the most likely position of the gene for a given marker order. The slick locus was confirmed to chromosome 20 with a maximum LOD score of 10.5 at 52 cM. Detailed haplotype inspection in the slick cows revealed a region where genotypes were conserved at three adjacent marker loci – DIK2416 (53 cM), DIK2930 (55.1 cM) and MB086 (56.6 cM). Results from genotyping this region with additional markers across more families, including the effect of this haplotype on Romosinuano (another slick hair coat breed) cattle will also be presented. The availability of a tightly conserved haplotype block will enable the genotyping of breeding stock for the slick hair gene. The potential benefits of introgressing such a gene associated with heat stress into the Holstein breed would be substantial for dairy production throughout the warmer regions of the world.