|Wiens, Gregory - Greg|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2004
Publication Date: 6/14/2005
Citation: Wiens, G.D., Strom, M.S. 2005. Estimation of the genome size of renibacterium salmoniarum atcc 33209 by pulsed-tield gel electrophoresis. (Abstract). 30th Eastern Fish Health Workshop. p. 8.
Technical Abstract: Renibacterium salmoninarum is a salmon and trout pathogen that belongs to the high G + C subgroup of gram-positive bacteria. Little is known about the molecular genetics of this microorganism. Here, we used pulsed-field gel electrophoresis to estimate the genome size of the ATCC 33209 type strain. Digestion of genomic DNA with PmeI generated four bands containing five restriction fragments with a cumulative size of 3.4 + .1 Mb. Seven previously identified Renibacterium genes were mapped to the PmeI fragments by Southern blotting. Both hly (metalloprotease) and one copy of msa (major soluble antigen or p57) were mapped to PmeI fragment I, while the other copy of msa, lysB and three IS994 insertion-element flanking-genes were mapped to PmeI fragments II/III. Unlike the duplicated msa gene, only single copies of hly, lysB and the three IS994 insertion-element flanking-genes were identified by Southern blotting. In summary, these data suggest that the genome size of R. salmoninarum ATCC 33209 is close to the average for prokaryotes (3.2 Mb), and that most R. salmoninarum genes are not duplicated.