|Stone, Andrew - Andy|
|Luster, Douglas - Doug|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/28/2005
Publication Date: 8/5/2005
Citation: Damsteegt, V.D., Stone, A.L., Sherman, D.J., Schneider, W.L., Gildow, F., Luster, D.G. 2005. In planta interactions of three plum pox potyvirus strains within cultivar Lovell peach seedlings[abstract]. Phytopathology. 95:S22.
Technical Abstract: Plum pox potyvirus (PPV) exists as several distinct strains: Dideron (D), Marcus (M), El Amar (EA), and Cherry (C). The M strain is considered the most easily aphid transmissible and epidemic form in peach. The D strain is the oldest recognized strain, most widely distributed worldwide, and the only strain found in the U. S. Very little is known about the interactions among strains when co-infecting the same tree seedlings. Utilizing the green peach aphid (Myzus persicae) as the vector, we inoculated replicated sets of Lovell peach seedlings with the M, D, and EA strains of PPV, singly, and in all combinations, simultaneously and sequentially with a three-week period between strain inoculations. Symptoms were recorded as they appeared and test plants were sampled periodically and new growth analyzed by ELISA, PCR, and subsequent aphid transmission. A multiplex PCR assay was developed to determine the presence of each strain in various tissues before and following vernalization.