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Title: CONSTRUCTION OF BAC AND FULL-LENGTH CDNA LIBRARIES FOR GENOMIC ANALYSIS OF THE WHEAT STRIPE RUST PATHOGEN (PUCCINIA STRIIFORMIS F. SP. TRITICI)

Author
item Ling, Peng
item Chen, Xianming
item LI, DAT - WASHINGTON STATE UNIV
item Garland-Campbell, Kimberly

Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 12/1/2004
Publication Date: 1/1/2005
Citation: Ling, P., Chen, X., Li, D.Q., Garland Campbell, K.A. 2005. Construction of bac and full-length cdna libraries for genomic analysis of the wheat stripe rust pathogen (puccinia striiformis f. sp. tritici). Plant and Animal Genome Abstracts. Vol 13, pg 94.

Interpretive Summary:

Technical Abstract: Puccinia striiformis Westend. f. sp. tritici causes stripe rust, one of the most destructive diseases of wheat worldwide. The genomic information of the fungus is currently unavailable. To determine sequence and genomic structures of the fungus and identify genes involved in its pathogenicity and biological development, we constructed a HindIII BAC library and a full-length cDNA library using urediniospores of race PST-78 of P. striiformis f. sp. tritici that has been predominant throughout North America. The BAC library consists of 22,272 clones with an average insert size of 60 kb, and was estimated to have a 10X coverage of the genome. A method using small amount of urediniospores to generate sufficient amount of HMW DNA was developed. The BAC clones will be used to generate whole genome physical map for the fungus. The full-length cDNA library consists of 42,240 clones, and 99% of the clones reached full-length with the average cDNA insert of 1.5kb. The functional analysis was initiated by sequencing 200 randomly selected cDNA clones. The sequences were compared with the accessions in the fungal gene database of the GenBank and categorized according to their putative functions. Genes like beta-tubulin, deacetylase, glyoxyloxidase, hydrophobin and mitigen-activated protein kinase were identified. These genetic resources and current results will serve as genomic platforms and starting points for the further studies of the P. striiformis genome.