Submitted to: Alfalfa Improvement Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 6/8/2004
Publication Date: 8/5/2004
Citation: Vandemark, G.J., Hughes, T.J., Larsen, R.C. 2004. Genetic similarities between alfalfa cultivars based on an analysis using sequence related amplified polymorphism (SRAP) DNA markers. Abstracts of the 39th North American Alfalfa Improvement Conference. A39.
Technical Abstract: Sequence related amplified polymorphisms (SRAPs) are relatively simple and highly reproducible DNA markers that are useful for both mapping and gene tagging in plants. DNA was isolated from commercial alfalfa cultivars and nine sources of alfalfa germplasm: Ladak, M. varia, Chilean, Peruvian, Indian, Turkestan, Flemish, African, and M. falcata. The DNA samples were amplified using 38 different SRAP primer pairs. Amplifications were performed in 25'l reactions containing 50 ng DNA, 200'M each dNTP, 1.5 mM MgCl2, 2.5 U Amplitaq Gold DNA polymerase, and 37 ng each forward and reverse primer. Polymorphisms were resolved on 15% polyacrylamide-TBE gels. Primer pairs varied considerably in the number of amplicons produced and in detectable polymorphisms. The 38 primer pairs produced over 400 amplicons. The nine sources of germplasm could all be clearly discriminated using as few as four different primer pairs. Currently we are using SRAPs to detect variation both within and between alfalfa cultivars representing a wide range of parental materials and zones of adaptation. The markers should be quite useful for linkage mapping and marker-assisted-selection in alfalfa.