|Miklas, Phillip - Phil|
Submitted to: American Society for Virology Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 3/31/2004
Publication Date: 7/10/2004
Citation: Larsen, R.C., Miklas, P.N., Druffel, K.L. 2004. Evidence of a genomic recombination event between a strain of bean common mosaic virus and bean common mosaic necrosis virus. American Society for Virology Meeting, July 10-14, 2004, Montreal, Canada. P40-11.
Technical Abstract: A virulent isolate of BCMNV was discovered in Kimberly, ID and was initially determined by ELISA and dry bean host range studies to be the NL3 strain. The isolate, designated NL-3 K, and the standard Drijfhout strain (NL3-D) both reacted similarly across bean host group differentials in most cases, although there were some distinct differences. Characteristics of NL-3 K included more severe symptoms with earlier onset on some bean lines. Symptoms of severe mosaic, stunting, and plant death occurred on beans from Host Group 0 while severe mosaic and systemic vein necrosis occurred on plants from host groups 3 and 10, respectively. The NL-3 K isolate also differed from NL3-D by producing necrotic local lesions followed by systemic vein necrosis on some bean lines containing the <I>I</I> + <i>bc-3</I> resistance genes. Deduced amino acid sequence data obtained near the 5' terminal of the P1 region revealed the NL-3 K strain to be an apparent genomic recombinant between NL3-D and the blackeye cowpea strain of BCMV. The first 109 amino acids at the N-terminal of the P1 protein were 86% identical with the cowpea strain. Following a transition region five amino acids in length beginning at position 110, the remaining 3134 amino acids of NL-3 K shared a 99% identity with the NL3-D strain. The genome length of NL-3 K was increased by 293 nucleotides when compared to NL-3 D. PCR primers designed to flank the recombination event of NL-3 K produced amplification products from the NL-3 K strain but not from bean infected only with NL-3 D or the BCMV cowpea strain. Similarly, primers specific to NL-3 D and BCMV-cowpea strain amplified products only from the respective virus-infected plants and not from NL-3 K. This is the first reported evidence of genomic recombination between strains of BCMV and BCMNV.