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Title: CROSS-REACTIVITY OF SIX ZEARALENONE ANTIBODIES IN A HAND-HELD FLUORESCENCE POLARIZATION IMMUNOASSAY

Author
item Maragos, Chris
item KIM, EUN-KYUNG - FORMER ARS, PEORIA, IL

Submitted to: International Union of Pure and Applied Chemistry
Publication Type: Proceedings
Publication Acceptance Date: 12/2/2004
Publication Date: 1/1/2006
Citation: Maragos, C.M., Kim, E. 2006. Cross-reactivity of six zearalenone antibodies in a hand-held fluorescence polarization immunoassay. International Union of Pure and Applied Chemistry XIth Meeting. p. 91-100.

Interpretive Summary:

Technical Abstract: Fusarium graminearum is a fungus that causes ear rot in maize and produces toxic metabolites (mycotoxins). One such mycotoxin is zearalenone, which has estrogenic activity. Contaminated feed can cause illness in livestock, particularly swine. Testing of commodities for zearalenone is currently done with enzyme-linked immunosorbent assays (ELISAs) or expensive chromatographic methods. Previously we have reported on the use of a fluorescence polarization immunoassay for detection of zearalenone in maize. The current report describes improvements to the assay including the use of a hand-held device and the comparison of five new zearalenone antibodies. This fast, easy to use, and low cost assays provides an additional tool for the rapid screening of commodities for zearalenone.