Submitted to: National Cotton Council Beltwide Cotton Conference
Publication Type: Proceedings
Publication Acceptance Date: 1/5/2004
Publication Date: 1/5/2004
Citation: Palmer, M.B., Main, D., Frelichowski, J., Tomkins, J.P., Ulloa, M. 2004. High-throughput development of new molecular markers for cotton. National Cotton Council Beltwide Cotton Conference. p. 1131.
Technical Abstract: The ubiquity of simple sequence repeats (SSRs) in eukaryotic genomes and their usefulness as genetic markers is now well established. A recent advance in molecular marker development has been the mining of simple sequence repeat SSR) markers from BAC-end sequence data. SSRs are desirable for use in marker systems because they are highly polymorphic, abundant, widely dispersed throughout eukaryotic genomes, transportable between laboratories, and are inherited in a codominant fashion. This approach provides a rich and novel source of new PCR-based markers, and the markers that are identified can be traced back to specific large-insert clones in the public cotton BAC library. This advantage has further applications towards map-based cloning and physical mapping of the entire cotton genome. Here we report on the development of SSRs from random sequencing of BAC clones from a large-insert library derived from the elite Upland cultivar Gossypium hirsutum L. Maxxa.