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United States Department of Agriculture

Agricultural Research Service

Title: CHARACTERIZATION AND MANIPULATION OF GENES IN THE BIOSYNTHESIS OF SOPHOROLIPIDS AND POLY(HYDROXYALKANOATES))

Author
item Solaiman, Daniel - Dan
item Ashby, Richard - Rick
item Foglia, Thomas

Submitted to: UJNR Food & Agricultural Panel Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 10/15/2004
Publication Date: 12/11/2004
Citation: Solaiman, D., Ashby, R.D., Foglia, T.A. 2004. Characterization and manipulation of genes in the biosynthesis of sophorolipids and poly(hydroxyalkanoates). Proceedings of the United States-Japan Cooperative Program in Natural Resources, Protein Resources Panel Annual Meeting. p. 215-219.

Interpretive Summary:

Technical Abstract: Agricultural fats and oils and their coproducts are economically and functionally attractive renewable feedstocks for the fermentative production of industrial bioproducts. Our laboratory has studied the use of these feedstocks for the production of biosurfactants (i.e., sophorolipids and rhamnolipids) and biodegradable polymer (i.e., poly(hydroxyalkanoates)). A genetic manipulation approach is pursued to effect the synthesis of new product variants and to improve production yields. In this paper, we present the identification, characterization and cloning of putative genes involved in the biosynthesis of sophorolipids. We also summarize results of our studies on the modification of genes involved in PHA synthesis and degradation, leading to the construction of recombinant strains with altered PHA composition and accumulation property.

Last Modified: 8/24/2016
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