|VIANNA, M C B|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 8/27/2004
Publication Date: 9/7/2004
Citation: Dubey, J.P., Sreekumar, C., Knickman, E., Miska, K.B., Vianna, M., Kwok, O.C., Hill, D.E., Jenkins, M.C., Lindsay, D.S., Greene, C.E. 2004. Biologic, morphologic and molecular characterization of neospora caninum isolates from littermate dogs (Abstract). COST 454 Annual Conference, Protozoal Reproduction Losses in Farm Ruminants. p.1.
Technical Abstract: Isolation and biologic and molecular attributes of Neospora caninum from three littermate dogs are described. Tissue cysts were confined to the brain and striated muscles. Neospora caninum was isolated (isolates NC-6, NC-7, and NC-8) in rodents and cell culture that had been inoculated with brain tissue from the dogs. Schizont-like stages reactive with N. caninum antibodies were seen in cell cultures seeded with bradyzoites released from Percoll-isolated N. caninum tissue cysts from the brain of one dog. Tissue cysts were infective orally to mice and gerbils, but not to cats and dogs. The isolates were also identified as N. caninum by PCR and sequence analysis.