Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 7/15/2005
Publication Date: 8/15/2005
Citation: Peng, C., Ferreira, J.F., Wood, A.J. 2005. Comparative analysis of artemisinin and derivatives by evaporative light scattering detection (HPLC-ELSD) and flame ionizatin detection (GC-FID). Poster Presentation, International Conference Quality and Safety Issues Related to Botanicals, National Center for Natural Products Research, School of Pharmacy, The University of Mississippi, August 15-18, 2005, University, MS.
Technical Abstract: Since the isolation and structural elucidation of artemisinin 32 years ago, artemisinin has been analyzed by different chromatographic techniques, including thin-layer chromatography (TLC), HPLC, GC, radioimmunoassay, and enzyme immunoassay. Due to the lack of chromophores, artemisinin is suitable for HPLC with UV detection only after derivatization. In the past, GC-FID analysis has been discarded due to the overlapping of one of the peaks generated by arteannuin B (a precursor) with one of the artemisinin degradation peaks, and HPLC with electrochemical detection (HPLC-EC) has been the choice method of analysis. This work compared the analyses of artemisinin, dihydroartemisinin, arteannuin B, and artemisinic acid from crude plant samples by GC with flame ionization detection (GC-FID) and HPLC with evaporative light scattering detector (HPLC-ELSD). Artemisia annua plants were field grown in Illinois, dried under 40 ºC, and extracted with petroleum ether. GC-FID and HPLC-ELSD were chosen due to their low cost compared to GC or HPLC coupled with mass spectrometry, and to their ease of operation compared to HPLC with electrochemical detection. Both GC-FID and HPLC-ELSD provided sensitive (ng level) and reproducible results when compared for the analysis of artemisinin, the main compound found in plants field-grown in Southern Illinois. The methods had a correlation coefficient of r2= 0.87, significant at p=0.001.