Submitted to: Journal of Animal Science Supplement
Publication Type: Abstract Only
Publication Acceptance Date: 4/15/2004
Publication Date: 6/1/2004
Citation: Richardson, R.L., Barb, C.R., Lee Rutherford, L., Kraeling, R.R., Hausman, G.J., Rekaya, R. Ontogeny of ovarian gene expression in the prepuberal pig. Journal of Animal Science Supplement. 2004. v. 82. suppl. 1. p. 65.
Technical Abstract: To determine molecular mechanisms that regulate ovarian development in the pig a custom microarray was developed to profile differential gene expression. Oligonucleotides (70 er) were produced from sequenced ESTs from the Meat Animal Research Center, ARS, USDA libraries that had at least 90% homology to known genes in The Institute of genome Research pig gene index. Total ovarian RNA was isolated from gilts at 90, 150 and 210 days (d) of age and used to prepare dye labeled cDNA probes, which were hybridized to arrays representing about 600 pig genes involved in growth and reproduction. Quantitative analysis using a mixed linear model statistical program identified (P<0.01) 41 genes differentially expressed from 90 to 210 d of age, which included genes involved in lipid/steroid metabolism, cell growth and regulation, and extracellular matrix adhesion. The gene, protein kinase-c theta, involved in apoptosis was up-regulated at 210 d. The transcription factor, PRDM2, was up-regulated at 90 and 150 d, but down regulated at 210 d. One unknown gene was down-regulated at 90d and up-regulated by 210 d. A number of these differentially regulated genes which were expressed in the pig ovary had not been reported in the human, including transcription factors, ATP binding cassette B member 11 and CCAAT/enhancer binding protein alpha. These results demonstrate, for the first time, differentially expressed ovarian genes during development in the prepuberal pig.