Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2003
Publication Date: 8/9/2003
Citation: Zhang, Q., Melcher, U., Bruton, B.D., Pair, S.D., Mitchell, F., Fletcher, J. 2003. Genomic comparison of plant pathogenic and non-pathogenic Serratia marcescens strains using suppression subtractive hybridization [abstract]. Phytopathology. 93:S94.
Technical Abstract: The causal agent of cucurbit yellow vine disease (CYVD) was identified as Serratia marcescens (Sm). Phenotypic comparison of CYVD-associated and non-phytopathogenic Sm strains showed significant differences. We used suppression subtractive hybridization to identify genetic changes responsible for the differences. Sm strain Z01-A, from CYVD-affected zucchini, was the tester, and the rice endophyte IRBG 502, 90 percent of whose genome is homologous to that of Z01-A by DNA-DNA hybridization, was the driver. Forty-seven unique contigs were formed out of 73 tester specific sequences generated from subtraction. Significant homologies were detected by BLAST searches, including 7 known transposases, 4 adhesion related proteins, 2 transcription regulators and 13 metabolic enzymes. The prevalence of putative transposase sequences suggests that the pathogenicity related genes have been acquired from other sources.