Submitted to: Society of Invertebrate Pathology
Publication Type: Proceedings
Publication Acceptance Date: 8/6/2004
Publication Date: 8/6/2004
Citation: Afonso, C.L., Kutish, G.F. 2004. Genome sequencing and analysis [abstract]. Society of Invertebrate Pathology. Interpretive Summary: Abstract only.
Technical Abstract: Determination of the entire viral genome sequence can lead to a better understanding of virus biology. Assembly of complete genomic DNA sequence allows comprehensive prediction of genome structure, coding capacity, transcriptional regulation of gene expression, and protein function. Presence of genes of known function allows prediction of virion structure, virus replication strategies, and putative viral-host interactions. Complete genome phylogenetic analysis of nucleic acid and protein sequences allow more accurate evolutionary predictions. Available molecular biology and bio-informatics tools allow selection of multiple strategies and methodologies for genome sequencing, assembly and analysis. Discussion will include brief descriptions of strategies, steps and methods for sequencing, genome assembly and analysis applicable to small and large viral DNA genomes. Criteria and methods will be presented for whole genome comparison, gene finding, identification of gene families, and functional prediction. A general introduction to free and commonly available UNIX, PC or server based DNA analysis tools and available databases will be presented. Logistics, timelines, pitfalls, and bottlenecks will be discussed.