Submitted to: Plant and Animal Genome VX Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2003
Publication Date: 1/11/2003
Citation: Rexroad III, C.E. 2003. Development of molecular tools for genome research in rainbow trout. Plant and Animal Genome Abstracts. Abstract W18B. p.8.
Technical Abstract: Selective breeding has long been used for the genetic improvement of species for agriculture. At the NCCCWA, we are integrating genomics, quantitative genetics, and physiology in a selective breeding program for rainbow trout with the goals of 1) developing technologies that identify superior performing genotypes and 2) identifying superior performing fish (with respect to stress response, growth, reproduction, and disease resistance) for inclusion into a selective breeding program. Ideally the use of genome technologies requires the development of species specific molecular tools and reagents, which has been our recent focus. Molecular genetic resources created at NCCCWA include 1) a 10 X Male rainbow trout bacterial artificial chromosome library constructed from the Swanson (YY) clonal line 2) ~45,000 EST sequences from a normalized cDNA library constructed from brain, gill, liver, kidney, spleen, muscle, and brain tissues and 3) microsatellite markers from multiple origins for mapping on USDA and other reference families. The characterization of these resources will be the focus of this talk.