Submitted to: Genetics in Aquaculture Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 11/9/2003
Publication Date: 11/9/2003
Citation: Rexroad III, C.E. 2003. Development of genetic markers for striped bass (morone saxitilis) and rainbow trout (oncorhynchus mykiss). Genetics in Aquaculture Symp. VII. Nov 9-15, 2003. p.8.
Technical Abstract: The NCCCWA is developing molecular genetic markers to aid in the genetic improvement of cool and cold water species for aquaculture production efficiency. Through the construction of genetic maps, we aim to identify quantitative trait loci (QTL) harboring genes having large affects on economically important traits. Once QTL are identified, marker assisted selection (MAS) can be utilized in a selective breeding program to enhance future generations of fish for these phenotypes. We are employing the use of microsatellites as they are co-dominant, present in large numbers, and appear to be widely distributed throughout the genome. Sources of marker discovery include microsatellite repeat-enriched libraries (MRELs), bacterial artificial chromosome (BAC) libraries, and expressed sequence tags (ESTs). In the case of rainbow trout, the large volume of EST data from the TIGR rainbow trout Gene Index (RTGI) is being exploited to identify single nucleotide polymorphisms (SNP) in genes of interest. These SNPs are useful as they are associated with a gene and can potentially provide comparative mapping information from better studied species. These markers are being utilized in population genetic studies, parentage identification studies, and in the construction of genetic maps.