Submitted to: Society for Invertebrate Pathology Annual Meeting
Publication Type: Proceedings
Publication Acceptance Date: 7/30/2003
Publication Date: 7/30/2003
Citation: Kim, W., Burand, J.P., Afonso, C.L., Kutish, G.F., Lu, Z., Rock, D.L. 2003.Hz-2v genome analysis [abstract]. Society for Invertebrate Pathology Annual Meeting. P. 78. Interpretive Summary: Abstract only.
Technical Abstract: Hz-2V is a viral pathogen, which causes the sterility in Helicoverpa zea moths. Hz-2V is a non-occludded, enveloped, rod-shaped virus, which contains double stranded circular genomic DNA. The 231,621-bp viral genome has 115 putative open reading frames, and closely resembles Hz-1 virus in DNA sequence homology and genomic organization. The G+C content of Hz-2V is 41.9% with a coding density of one gene per 2kb. Sequence analysis using the GenBank database identified 29 open reading frames (ORFs), of which 18 ORFs showed significant homology to proteins of known function. Some of these identified ORFs are related to known genes involved in DNA replication, (DNA polymerase and ligase), RNA transcription (VLF-1, LEF-8), and apoptosis inhibition. Some baculovirus structural protein gene homologs were identified (pdv-e56, p74, and p91) while the Hz-2V structural protein genes (p11.7, p31.7) identified by mass-spectroscopy did not show significant homology to any known gene sequences. Interestingly, many homologs to cellular genes were also identified in the Hz-2V genome including carboxylesterase, ribonucleotidereductase, serine hydroxymethlytransferase, deoxyribonucleosidereductase, dihydrofolatereductase, and zinc metalloprotease from Drosophila, Ades and Heliothis. Hz-2V also contains several additional baculovirus gene homologues (AcORF-22, AcORF-98, AcORF-119, PxORF-109, and PxORF-29), however, the relationship between Hz-2V and baculoviruses based on gene content and organization, is still unclear.