Author
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Tabatabai, Louisa |
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Submitted to: Meeting Abstract
Publication Type: Abstract Only Publication Acceptance Date: 9/1/2003 Publication Date: N/A Citation: N/A Interpretive Summary: Technical Abstract: Commercial bacterins for controlling fowl cholera are comprised of P. multocida serotypes A:1, A:3, and A:4 and are most often associated with outbreaks of fowl cholera. A 39-kDa outer membrane protein, previously described as the protective factor protein of the outer membrane proteome, was identified as Pasteurella lipoprotein B, PlpB. The 3-({3-cholamidopropyl]-dimethylammonio)-1-propanesulfonate (CHAPS)-soluble proteome contained 11 major proteins. Five proteins were identified by peptide mass fingerprinting and MALDI-TOF mass spectrometry. Two of the proteins were associated with membrane biogenesis, lipase A (LipA/Phy A, Mr 79,739) and lipoprotein (Mr 37,374). Three proteins were identified as potential virulence factors, Lipoprotein B (PlpB, Mr of 30,233), Putative Virulence factor (Mr of 117,437), and Porphyrin Biosynthetic Protein (HemY, Mr of 47,773). The identity of PlpB, which has a molecular weight of 39-40 kDa by SDS-PAGE, was confirmed by N-terminal sequence determination of two C18-HPLC-purified peptides. Flanking proteins and their functions are also described. |
