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ARS Home » Research » Publications at this Location » Publication #151593


item Bailey, Joseph
item Cosby, Douglas

Submitted to: Journal of Food Protection
Publication Type: Abstract Only
Publication Acceptance Date: 8/31/2003
Publication Date: 11/1/2003
Citation: Bailey, J.S., Cosby, D.E. 2003. Detection of salmonella from chicken rinse and chicken franks with electrochemiluminescence and automated pcr assays.. Journal of Food Protection.

Interpretive Summary:

Technical Abstract: Processed chicken carcass rinses were sampled for Salmonella after buffered peptone (BP) rinse or after the addition of either 3 or 0.3 Salmonella/mL of rinse. Chicken franks were cut into 10 g portions and stomached with 90 mL of BP and sampled for Salmonella directly or after the addition of either 31 or 3 Salmonella/mL. The entire experiment was replicated twice. All samples were tested for Salmonella by the cultural procedures of FSIS, by the IGEN PATHIGEN® Salmonella electrochemiluminescence procedure after 18-20 hr in BP and after an additional 24 hr in RV, and by the Qualicon BAX® automated PCR Salmonella assay after 18-20 hr in BP. Briefly, the The PATHIGEN Salmonella Test uses a sandwich immunoassay format in which a polyclonal antibody specific for Salmonella binds the organism to a paramagnetic microparticle and a second polyclonal antibody specific for Salmonella is labeled with a compound which is excited at the surface of an electrode and emits light when Salmonella is present. The PATHIGEN® takes about 2 hours to run. The automated BAX® assay procedure is as follows: overnight growth (16 to 18 hours) of sample in buffered peptone broth at 35 C, transfer of sample to lysis tubes, incubation and lysis of cells, and transfer of sample to PCR tubes which are placed in cycler/detector which runs automatically. The BAX® PCR/detection assay takes about 4 hours. From chicken rinse samples, Salmonella were recovered from all (14) high inoculum samples by all methods and from all but one of the low inoculum samples with the PATHIGEN from BP. From chicken franks, Salmonella were recovered from all (14) high inoculum samples by all methods and from 13 of 14 samples by the BAX and PATHIGEN as compared to 14 of 14 cultural samples. Both the PATHIGEN and BAX assays were easy to run and were highly effective alternative methods to the more time consuming standard cultural procedure.