Submitted to: Diseases at the Interface between Domestic Livestock and Wildlife Species
Publication Type: Abstract Only
Publication Acceptance Date: 7/3/2003
Publication Date: 7/17/2003
Citation: LEHMKUHL, H.D., HOBBS, L.A., WOODS, L.W. NEED FOR WILDLIFE SPECIFIC TOOLS: CHARACTERIZATION OF AN ADENOVIRUS THAT CAUSES HEMORRHAGIC DISEASE IN DEER. DISEASES AT THE INTERFACE BETWEEN DOMESTIC LIVESTOCK AND WILDLIFE SPECIES. 2003. Abstract. P. 22.
Technical Abstract: An adenovirus associated with a newly recognized hemorrhagic disease in deer in North America produces both systemic and localized lesions. Systemic lesions included pulmonary edema and less often hemorrhagic enteropathy. Vasculitis with endothelial intranuclear inclusion bodies was seen primarily in the lungs and alimentary tract and less frequently in the brain, kidney, spleen, pulmonary artery, and urinary bladder. Endothelial intranuclear inclusion bodies were seldom seen in deer with localized lesions. Adenovirus associated with systemic and localized vascular damage was demonstrated by transmission electron microscopy and immunohistochemistry. Early attempts to propagate the virus in a variety of cell cultures derived from bovine, caprine, and ovine fetuses failed. The production of fetal white-tail deer lung and turbinate cell cultures allowed for propagation of the virus. Because of the lack of vigor of these derived cell cultures, cervine cell culture adapted virus was used to inoculate low passage fetal bovine, caprine and ovine cells, but without success. Development of cervine cell cultures for virus propagation was crucial to virus characterization, development of immunologic based tests for prevalence assessments, development of molecular based tests, and assessment of pathogenicity of this adenovirus.