|Miklas, Phillip - Phil|
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 2/1/2004
Publication Date: 4/1/2004
Citation: Larsen, R.C., Miklas, P.N. 2004. Generation and molecular mapping of a scar marker linked with the bct gene for resistance to beet curly top virus in common bean. Phytopathology. 94:320-325.
Technical Abstract: Beet curly top virus (BCTV) is a persistent disease problem for bean production in the Pacific Northwest and other arid regions where the virus and aphid vector, Circulifer tenellus, are present. Breeding for resistance has been difficult because field screening usually results in sporadic infection across and within test plots. To circumvent the lengthy screening process required for identification of genetic resistance, a DNA marker tightly linked with a major BCTV resistance gene was developed with application for marker-assisted selection (MAS). Separate DNAs bulked from eight curly top-resistant recombinant inbred lines (RILS) and eight curly top-susceptible RILs were screened with 750 random decamer RAPD primers. A 1550 bp RAPD marker that selectively amplified products present in the resistant bulk DNAs was converted to a SCAR marker to facilitate high throughout MAS of the Ctv-1 resistance gene across breeding programs. The SCAR was located 1.1 cM from Ctv-1 in a population of 96 RILs. A survey of 70 cultivars and breeding lines revealed that this marker was for MAS of Ctv-1 in snap bean.