|Richard Jr, Edward|
Submitted to: Clemson University Vegetable Report
Publication Type: Abstract Only
Publication Acceptance Date: 5/20/2003
Publication Date: 6/20/2003
Citation: Pan, Y., Richard Jr, E.P. 2003. Microsatellite DNA Fingerprints of Sugarcane Clones and Their Implications in Sugarcane Breeding [abstract]. Sugar Journal. 66(1):27.
Technical Abstract: Microsatellites, also known as simple sequence repeats (SSRs) or short tandem repeats (STRs) are inheritable DNA markers that contain various numbers of tandem repeat units of di-, tri-, or tetra-nucleotide motifs. In this study, 25 Louisiana sugarcane clones, a Reunion clone R570, and an Australian clone Q124 were fingerprinted with nine sugarcane microsatellites using a capillary electrophoresis system. In total, 52 molecular alleles were identified. Eleven were common alleles found in all 27 clones. The remaining 41 alleles were, however, found in some but not all clones. There were eight alleles for the microsatellite SMC286CS, five for SMC334BS, eight for SMC336BS, four for SMC713BS, five for mSSCIR5, five for mSSCIR33, five for MCSA042E08, four for MCSA053C10, and eight for MCSA068G08. Presence or absence of these 52 alleles from a clone constituted a DNA fingerprint or genotype for that clone. The genetic relatedness among these clones was assessed according to their microsatellite fingerprints. There were three groups of clones that shared at least 73% pairwise identity between their microsatellite fingerprints. Group I included HoCP 96-509, HoCP 97-609, HoCP 98-718, HoCP 98-741, HoCP 96-540, HoCP 98-771, L 98-209, LCP 85-384, HoCP 98-778, L 97-128, HoCP 98-776, HoCP 85-845, HoCP 97-606, CP 72-370, L 98-207, LCP 86-454, and HoCP 98-734. Group II included HoCP 91-555, L 95-462, LCP 82-89, HoCP 98-781, and R570. Group III included CP 70-321, CP 65-357 and LHo 83-153. Two clones, L 97-137 and Q124, were clustered outside the three major groups. The results from this study indicates that a narrow genetic background is shared among the current Louisiana clones illustrating the need to broaden their genetic base.