Submitted to: Cytogenetics and Genome Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/1/2003
Publication Date: 12/1/2003
Citation: Rexroad III, C.E., Gahr, S.A., Palti, Y., Karamycheva, S., Keele, J.W., Quackenbush, J. 2003. Sequence analysis of a rainbow trout cdna library and creation of a gene index. Cytogenetics and Genome Research. 102(1-4):347-354. Interpretive Summary: Genetic research in any species is facilitated by large amounts of gene sequence data. This manuscript describes our efforts to obtain gene sequence data from rainbow trout brain, gill, liver, kidney, spleen, and muscle tissue in order to identify as many unique genes as possible. We compared our results with previously characterized genes from other species to associate them with a known function.
Technical Abstract: Expressed sequence tag (EST) projects have produced extremely valuable resources for identifying genes affecting phenotypes of interest. A large scale EST sequencing project for rainbow trout was initiated to identify and functionally annotate as many unique transcripts as possible. Over 45,000 five prime ESTs were obtained by sequencing clones from a single normalized library constructed using mRNA from six tissues. The production of this sequence data and creation of a rainbow trout Gene Index eliminating redundancy and providing annotation for these sequences will facilitate research in this species.