Submitted to: Federation Of European Microbiological Societies Yeast Research
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/25/2003
Publication Date: 9/1/2003
Citation: Kurtzman, C.P. 2003. Phylogenetic circumscription of Saccharomyces, Kluyveromyces and other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora. Federation of European Microbiological Societies Yeast Research. 4:233-245. Interpretive Summary: Yeasts assigned to Saccharomyces, Kluyveromyces, Zygosaccharomyces and other genera of the "Saccharomyces complex" represent the world's major industrial microorganisms (bread, alcoholic beverages, fuel alcohol, recombinant DNA products), and also include major food spoilage organisms. Identification of these organisms has been difficult using traditional methods. This research reclassifies the ca. 80 species in the "Saccharomyces complex" based on analysis of multiple gene sequences. The reclassification now makes it possible to rapidly identify species and genera directly from gene sequences or from molecular probes based on these gene sequences.
Technical Abstract: Genera currently assigned to the Saccharomycetaceae have been defined from phenotype, but this classification does not fully correspond with species groupings determined from phylogenetic analysis of gene sequences. The multigene sequence analysis of Kurtzman and Robnett (FEMS Yeast Res. in press) resolved the Saccharomycetaceae into 11 well supported clades. In the present study, the taxonomy of the Saccharomyctaceae is evaluated from the perspective of the multigene sequence analysis, which has resulted in reassignment of some species among currently accepted genera, and the proposal of the following five new genera: Lachancea, Nakaseomyces, Naumovia, Vanderwaltozyma and Zygotorulaspora.