|Van Tassell, Curtis - Curt|
Submitted to: Animal Production World Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 7/10/2003
Publication Date: 7/10/2003
Citation: Mota, A.F., Sonstegard, T.S., Van Tassell, C.P., Shade, L.L., Matukumalli, L., Wood, D.L., Capuco, A.V., Brito, M.A., Martinez, M.L., Connor, E.E. 2003. Characterization of ORESTES cDNA libraries generated from mammary gland tissues of Holstein (Bos taurus) and Gir (Bos indicus) cattle [abstract]. Animal Production World Conference Proceedings.
Technical Abstract: Currently, there is approximately an order of magnitude less sequence information in public databases than that available for characterizing the transcriptomes of human and mouse. Furthermore, there is a complete lack of sequence information from Bos indicus (zebu) breeds, which dominate the large cattle markets of tropical production areas. Generating expression sequence tags (EST) from zebu cattle is important to better characterize bovine genetic diversity related to health and production and to more thoroughly identify, annotate and classify bovine genes. This information will be essential to the interpretation of results generated from functional genomic studies. We used a modification on the strategy to produce EST, named ORESTES (Open Reading Frame ¿ORF EST). We deposited 6,481 EST in public databases using healthy and infected involuted mammary gland tissues from Holstein and mature Brazilian Dairy Gir cows (zebu). Alignment and assembly of the 6,481 EST generated 1,701 unique sequence elements, and of these, 1,089 did not significantly match sequence assemblies available in the Bos taurus gene index, with a threshold of e=10x10-10 and score=100. However, a total of 683 of these 1,089 sequence elements did comparatively align with mouse and human EST demonstrating these libraries could be used to efficiently discover novel sequences. Furthermore, comparison of the alignment position of all bovine sequence elements matching human full-length reference cDNA sequences suggested that the majority of arbitrarily priming events for cDNA synthesis occurred towards the central portion of the transcripts, which may be correlated to the relatively high rate of novel sequence discovery.