Submitted to: Biotechnology for Fuels and Chemicals Symposium Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/7/2003
Publication Date: 5/7/2003
Citation: EBENER, J.M., QURESHI, N., EZEJI, T.C., BLASCHEK, H.P., DIEN, B.S., COTTA, M.A. CORN FIBER HYDROLYSIS AND FERMENTATION TO BUTANOL USING CLOSTRIDIUM BEIJERINCKII BA101. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. PAPER NO. 2-14.
Technical Abstract: Corn fiber was hydrolyzed by dilute sulfuric acid treatment and enzymes by a method reported by Grohmann and Bothast (1997). Sulfuric acid concentration of 0.3-0.5% resulted in better hydrolysis than 0.1-0.2%. Sixty-three g/L corn fiber (16% moisture content) were treated with sulfuric acid [0.3-0.5% (v/v)] at 121 deg C for 1 h followed by cooling to room temperature and adjusting pH to 4.5 with 10 M NaOH. The hydrolysate so obtained contained approximately 30 g/L total sugar. Subsequent hydrolysis with enzymes [cellulase and cellubiase, both at 1 mL/100 g corn fiber] increased sugar concentration to approximately 52 g/L. There was no effect of reduced particle size on the hydrolysis and final sugar content in the hydrolysate. The hydrolysate was used to measure cell growth of Clostridium beijerinckii BA101 in anticipation for producing butanol. Compared to the control, reduced cell growth was observed, possibly due to growth inhibitory components present in the hydrolysate. The results on hydrolysis and butanol fermentation will be reported during the 25th Biotechnology Conference on Fuels and Chemicals.