Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/11/2003
Publication Date: 1/11/2003
Citation: Reddy, U.K., Padmavathi, N., Saha, S., Katam, R., Saha, S., Jenkins, J.N., Thomas, C.E., Levi, A. 2003. Di and tri nucleotide repeat motifs for marker development in watermelon. Plant and Animal enome Conference Proceedings. p. 182. Interpretive Summary: Not required.
Technical Abstract: Repeat motifs in the genome, known as SSRs (Simple Sequence Repeats) or microsatellites, can be easily exploited as potential co-dominant markers using modern PCR technologies. Saturation of watermelon genetic maps with the SSRs will speed up mapping and further isolation of genes that are governing important traits. To start with, we captured large numbers of fragments containing microsatellites from genomic DNA of New Hampshire Midget (NHM). The capturing method involved hybridization of biotin labeled synthetic oligoes containing different types of repeats to digested genomic DNA fragments. Sequences of 100 of these DNA fragments were determined. Several repeat types were represented including GA, CA, AGA and ACA. Primers were designed and tested on mapping parents. Polymorphism among various watermelon types and the potential to map these will be discussed.