Submitted to: Research Workers in Animal Diseases Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 9/23/2002
Publication Date: 10/20/2003
Citation: TABATABAI, L.B. THE 39 KDA PROTEIN OF PASTEURELLA MULTOCIDA CROSS-PROTECTIVE FACTOR IS THE OUTER MEMBRANE LIPOPROTEIN B, PLPB. CONFERENCE OF RESEARCH WORKERS IN ANIMAL DISEASES, PROCEEDINGS OF THE 83rd ANNUAL MEETING. 2003. Abstract #151.
Technical Abstract: Commercial bacterins for the control of fowl cholera are comprised of P. multocida serotypes A:1, A:3, and A:4, which are the serotypes that are most often associated with outbreaks in poultry flocks. The 39 kDa antigen was previously isolated from in vivo-grown P. multocida and was found to induce active immune cross protection and passive immune cross protection depending on the dose administered. An outer membrane protein extract containing the 39 kDa was submitted to denaturing 1-dimensional sodium dodecyl electrophoresis. The 39 kDa protein was identified from a plot of molecular weight versus mobility and excised from the Coomassie Brilliant Blue-stained gel. The excised band was digested with trypsin, and the peptides purified by reverse-phase HPLC. Selected peptide peaks were analyzed by N-terminal sequencing, MALDI-TOF mass spectrometry, and database analysis using MS-FIT. The MS-FIT data were confirmed by N-terminal sequence information for two peptides of the digest. The results indicated that the 39 kDa cross protective antigen is the P. multocida lipoprotein B, PlpB.