Submitted to: Meeting Abstract
Publication Type: Proceedings
Publication Acceptance Date: 10/1/2002
Publication Date: 6/1/2003
Citation: KINGSLEY, D.H. MOLECULAR DETECTION AND HIGH PRESSURE SANITIZATION OF SHELLFISH-BORNE VIRUSES. MEETING ABSTRACT. 2003. p. 475-483.
Technical Abstract: A highly sensitive virus RNA extraction method for shellfish, using Glycine buffer, Polyethylene glycol, Tri-reagent and poly dT magnetic beads, termed the GPTT procedure, has recently been developed which facilitates RT-PCR detection of hepatitis A (HAV), hepatitis E (HEV), and Norwalk-like viruses (NLV). This procedure detects less than one plaque-forming unit of HAV and approximately 22.4 RT-PCR units of NLV. It has been successfully adapted to detect both hepatitis A and Norwalk-like viruses within clams implicated in an outbreak of viral gastroenteritis within the United States. High hydrostatic pressure processing, a new commercial technology that readily inactivates Vibrio bacteria within shellfish meats while retaining uncooked characteristics, has been evaluated for the inactivation of HAV and feline calicivirus (FCV), a Norwalk virus surrogate. We demonstrated that 5-min exposures at 450 megaPascals (approximately 4500 atm) are sufficient to inactivate a 7-log10/ml titer of HAV. Dilution of HAV in seawater, however, reduced inactivation of the virus by pressure. For FCV, we demonstrated inactivation of a 7-log10/ml titer with 275 MPa of pressure for 5 min.