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ARS Home » Research » Publications at this Location » Publication #133215
Title: PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES DETECTING THE CHICKEN INTERLEUKIN-1 RECEPTOR ALPHA CHAIN

Author
item LI, GUANGXING - 1265-40-00
item Lillehoj, Hyun
item MIN, WONGI - 1265-40-00

Submitted to: Poultry Science Association Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/1/2002
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: We recently cloned the genes of the chicken IL-15 and IL-15 receptor (R) a proteins. In this study, twelve monoclonal antibodies against recombinant chicken IL-15Ra were produced and characterized. By enzyme-linked immunosorbent assay, all mAbs showed binding specificity for IL-15Ra but not IL-2 or interferon-g and identified a 25.0 kDa protein by immunoblot analysis. Flow cytometric analysis revealed negligible expression of IL-15Ra on nonactivated lymphocytes from the spleen, thymus or bursa, low but detectable expression on macrophages and high expression on concanavalin A-activated spleen lymphoblasts. Established chicken T cell (RP13) and macrophage (HD11) cell lines expressed substantially higher levels of IL-15Ra compared with a B cell line (RP9). Two mAbs inhibited IL-15 dependent proliferation of T cells suggesting that the tertiary structure of the protein domain of native IL-15Ra that binds to IL-15 is preserved in the recombinant receptor molecule. These mAbs will be useful reagents for further in vitro and in vivo studies of the biological functions of chicken IL-15 and its receptor.