|LI, W - HUBEI UNIV, CHINA
|DERY, PIERRE - USDA, RETIRED
Submitted to: Phytopathology
Publication Type: Abstract Only
Publication Acceptance Date: 7/30/2002
Publication Date: N/A
Technical Abstract: Identification of genes involved in seed and root colonization by biocontrol bacteria may allow for strategies for improved ecological fitness, survival, and performance by these strains. Enterobacter cloacae strain M59, containing a single transposon insertion, was reduced in colonization of cucumber seeds relative to wild-type strain 501R3 but unaffected in control of Pythium ultimum damping-off on cucumber. DNA sequence analysis indicated that the transposon was inserted in cyaA, which encodes adenylate cyclase. The growth profile of strain M59 was consistent with a cyaA mutant. Adenylate cyclase catalyzes the production of cAMP, which in conjunction with CRP, positively regulates several catabolic and other unrelated functions.