Submitted to: International Congress of Mycology Proceedings and Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 8/2/2002
Publication Date: N/A
Technical Abstract: Ninety isolates of Penicillium spp. collected from decaying carpophores of macro fungi originating in North America and about 30 isolates from culture collection sources were examined phenotypically and genetically to identify the isolates and to correlate phenotype with genotype. ITS1, 5.8S, ITS2 and partial 28S rDNA (total, ca 1150 nt) were amplified as a single fragment and the DNA was sequenced. Sequences were compared to homologous sequences from ex-type cultures (Peterson, 2000). Perfect match or 1 nt difference was taken to represent the same species, while greater differences in the sequences represented evidence that the species were genetically distinct. We discovered more than 30 new species of Penicillium. In some species, the phenotype was distinctive and correlated well with the genotype. In other cases, the phenotypic and genotypic identifications were different. One genetic group of isolates, centered around Penicillium sclerotiorum, was studied using a multi-locus approach to identify genetically isolated groups. This model study suggests that sequences from the 1150 nt ITS-28S region are predictive of species-level genetic isolation among Penicillium isolates. Some of the newly discovered isolates have been examined for secondary metabolite production, and we have found that several new species produce novel compounds.