Submitted to: HortScience
Publication Type: Abstract Only
Publication Acceptance Date: 12/5/2000
Publication Date: 6/15/2001
Citation: Wang, S.Y., Cregan, P.B., Jiao, H., Fickus, E.W., Jones, R.W., Hokanson, S.C. 2001. Abundance of simple sequence repeats (microsatellites) in strawberry.. Hortscience.
Technical Abstract: Simple Sequence Repeat (SSR) DNA markers, also known as microsatellites or Short Tandem Repeats (STR), have been used successfully for the purpose of genotype identification in plants; however, no SSR research has previously been performed in strawberry. We screened the genomic DNA from a variety of strawberry genotypes including Fragaria x ananassa ('Earliglow'), F. virginiana ['Fra23' (RH23)], F. chiloensis ('Pigeon Point'), and F. vesca (virus indicator UC5), to determine the abundance of several di-, tri-, and tetrameric SSR motifs. The DNA samples were digested with EcoR I, Rsa I and Hinf I, and labeled (32P) oligonucleotide probes complementary to di-, tri-, and tetrameric repeats were hybridized to Southern blots of digested DNAs. Various SSRs were evident among the different genomic DNA samples tested. Among the di-nucleotide motifs, (CA/GT)n and (CT/GA)n appeared to be the most abundant repeats. With regards to tri-nucleotide motifs, (GGA/CCT)n and (GAA/CTT)n were apparently the most abundant repeats, followed by (CAG/GTG)n, (CAA/GTT)n, and (CGT/GCA)n. Meanwhile (TAA/ATT)n, (CGA/GCT)n, (ATG/TAC) and (GAT/CTA)n were the least common repeats in strawberry genomic DNA. Limited hybridization of the tetranucleotides, (GATA/CTAT)n, (TAGA/ATCT)n, and (GAAT/CTTA)n, was observed, possibly due to their low frequency in the genomes examined.