Submitted to: Proceedings International Rice Genetic Symposium
Publication Type: Proceedings
Publication Acceptance Date: 10/19/2000
Publication Date: N/A
Technical Abstract: A microsatellite sequence at the waxy gene of rice explains much of the variation in apparent amylose content (AA) of non-waxy US germplasm. Since the amylose content of breeding lines is typically evaluated across multiple years and locations, this microsatellite shows good promise for marker aided selection of amylose content. The relationship between this microsatellite and AA using a diverse set of cultivars would elucidate how broadly useful this technology may be for breeding programs which use international germplasm. This study examined the association between the microsatellite and AA using approximately 200 hundred accessions from 53 countries. A previously unreported allele, (CT)10, was found in accessions that had AA in low, intermediate and high classes. A broader range in AA contents was identified for (CT)20, (CT)18, (CT)16 and (CT)8 alleles than was previously reported. The variance of AA explained by the microsatellite efor non-waxy accessions and known mutants was 68 percent. The results indicate that this microsatellite marker can be a useful method for predicting the AA content class of a diversity of rice germplasm. However, the relationship of AA content with this microsatellite can be confounded by production environment and analytical methods for determining AA, as well as other genes and mutations that may occur in the genome.