|Nourse Styan, Sarah|
Submitted to: North American Strawberry Conference Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2001
Publication Date: N/A
Technical Abstract: Simple sequence repeats are regions of the genome characterized by islets of repetitive -di, -tri, or -tetra-nucleotide sequences. DNA fragments containing these repeat motifs can be amplified via PCR using a pair of oligonucleotide primers specific to the regions flanking the repeat region. Polymorphisms are based on variation in the number of repeat units at the specific locus amplified and are visualized after electrophoresis on agarose or polyacrylamide gels. SSR markers are widely used because they are co-dominant, technically simple to use, highly polymorphic, and have been shown to be well distributed within the genomes of many plant and animal species. SSR markers for strawberry should be of great utility for many applications including, fingerprinting of strawberry cultivars and germplasm, genetic diversity assessment, QTL discovery, gene mapping, and marker assisted selection. Genomic DNA from the cultivar Earliglow was digested with Sma1, Rsa1, and Msc1 restriction enzymes, electrophoresed, and DNA fragments of 400-800bp were selected. The DNA fragments were ligated into pUC19 plasmid cloning vector and a genomic library was constructed. The library is being screened with P32 labeled repeat probes. To date we have identified 40 (CT)n and 4 (ATT)n repeat containing clones. We will continue with further genomic library screening, sequence analysis, and design of primers for PCR.