Submitted to: Crop Production and Protection Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 8/1/2000
Publication Date: 8/21/2000
Citation: Chenault, K.D., Burns, J.A., Melouk, H.A. 2000. Production and analysis of transgenic peanut plants containing anti-fungal genes [abstract]. Crop Production and Protection Conference Proceedings. 1p. Interpretive Summary:
Technical Abstract: Two anti-fungal genes, a rice chitinase and/or an alfalfa glucanase, were used to produce transgenic peanut (Arachis hypogaea L. cv. Okrun) by microprojectile bombardment of embryonic cultures. Independent transformation events resulted in the production of over 500 different transgenic peanut lines. Molecular analysis of fertile regenerated lines through the T3 generation has demonstrated stable inheritance patterns for the transgene(s) in 32 separate lines, as well as increased chitinase/glucanase activity ranging from 0 to 60% above background levels. Greenhouse studies in which the transgenic plants were challenged with the fungus Sclerotinia minor have shown that several of these lines show resistance levels equal to or exceeding those of S. minor-resistant cultivars currently being grown by producers.