Submitted to: Animal Genetics International Conference Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 3/14/2000
Publication Date: N/A
Technical Abstract: Micromolar calcium activated neural protease (CAPN1) was investigated as a potential candidate gene for a quantitative trait locus (QTL) on BTA29 affecting meat tenderness. A 2,948 base pair (bp) bovine cDNA containing the entire coding region of the gene was obtained, showing 91% identity to human CAPN1. The 716 amino acid (aa) protein predicted from this sequence shows 97% similarity (95% identity) to the 714 aa human protein. Analysis of the gene structure revealed that CAPN1 mRNA is encoded by at least 20 exons, and 9,800 bp of the gene were sequenced including 17 of the introns. Two single nucleotide polymorphisms (SNP) were detected in intron 12 and used to map bovine CAPN1 to the telomeric end of the BTA29 linkage group. This approximately coincides with the position of the QTL, demonstrating that CAPN1 protease is a positional candidate gene potentially affecting variation in meat tenderness in a bovine resource mapping population.