Submitted to: American Peanut Research and Education Society Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/11/2000
Publication Date: 12/1/2000
Citation: Chenault, K.D., Burns, J.A., Melouk, H.A. 2000. Production of transgenic peanut plants containing anti-fungal genes [abstract]. American Peanut Research and Education Society Abstracts. 32:70.
Technical Abstract: Two anti-fungal genes, a rice chitinase and/or an alfalfa glucanase, were used to produce transgenic peanut (Arachis hypogaea L. cv. Okrun) by microprojectile bombardment of embryonic cultures. Independent transformation events resulted in the production of over 500 different transgenic peanut lines. Molecular analysis of 32 fertile regenerated lines sthrough the T3 generation have demonstrated stable inheritance patterns fo the transgene(s) as well as increased chitinase/glucanase activity ranging from 0 to 75% above background levels. Greenhouse evaluations in which the transgenic peanut plants were inoculated with Sclerotinia minor, the causal agent of Sclerotinia blight, have indicated that several of these lines exhibited resistance levels equal to or exceeding those of S. minor- resistant cultivars currently being grown in commerce.