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ARS Home » Research » Publications at this Location » Publication #109937
Title: CHARACTERIZATION OF ANTIBODY RESPONSE TO PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS ORF5 PRODUCT FOLLOWING INFECTION AND EVALUATION OF ITS DIAGNOSTIC USE IN PIGS

Author
item KWANG, JIMMY - FORMER ARS EMPLOYEE
item YANG, SIMON - FORMER ARS EMPLOYEE
item OSORIO, FERNANDO - UNIVERSITY OF NEBRASKA
item CHRISTIAN, STEVE - UNIVERSITY OF NEBRASKA
item WHEELER, J - UNIVERSITY OF NEBRASKA
item LAGER, KELLY - 3625-30-20
item LOW, SHARON - UNIVERSITY OF SINGAPORE
item CHANG, LEO - BESTAR LAB., SINGAPORE
item DOSTER, ALAN - UNIVERSITY OF NEBRASKA
item WHITE, AMY - UNIVERSITY OF NEBRASKA

Submitted to: Journal of Veterinary Diagnostic Investigation
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/1/1999
Publication Date: N/A
Citation: N/A

Interpretive Summary: There is a need for improved diagnostic tests for porcine reproductive and respiratory syndrome (PRRS) virus infection in swine. This study compared a commonly used commercial test with a test based on a single protein expressed from a cloned PRRS gene, ORF 5. The new test exhibited increased sensitivity, as well as detecting infection approximately 1 week earlier, compared to the commercial test, indicating a significant improvement in diagnostic efficacy.

Technical Abstract: The sensitivity and specificity of recombinant open reading frame 5 products used in the western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n=30), 2) naturally infected pig sera (n=40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to three state diagnostic laboratories (n=200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the two assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies.