Author
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KWANG, JIMMY - FORMER ARS EMPLOYEE |
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YANG, SIMON - FORMER ARS EMPLOYEE |
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OSORIO, FERNANDO - UNIVERSITY OF NEBRASKA |
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CHRISTIAN, STEVE - UNIVERSITY OF NEBRASKA |
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WHEELER, J - UNIVERSITY OF NEBRASKA |
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LAGER, KELLY - 3625-30-20 |
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LOW, SHARON - UNIVERSITY OF SINGAPORE |
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CHANG, LEO - BESTAR LAB., SINGAPORE |
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DOSTER, ALAN - UNIVERSITY OF NEBRASKA |
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WHITE, AMY - UNIVERSITY OF NEBRASKA |
Submitted to: Journal of Veterinary Diagnostic Investigation
Publication Type: Peer Reviewed Journal Publication Acceptance Date: 8/1/1999 Publication Date: N/A Citation: N/A Interpretive Summary: There is a need for improved diagnostic tests for porcine reproductive and respiratory syndrome (PRRS) virus infection in swine. This study compared a commonly used commercial test with a test based on a single protein expressed from a cloned PRRS gene, ORF 5. The new test exhibited increased sensitivity, as well as detecting infection approximately 1 week earlier, compared to the commercial test, indicating a significant improvement in diagnostic efficacy. Technical Abstract: The sensitivity and specificity of recombinant open reading frame 5 products used in the western blotting assay for confirmation of porcine reproductive and respiratory syndrome virus (PRRSV) serologic status were evaluated. The recombinant antigen-based assays were specifically compared with a commercial enzyme-linked immunosorbent assay (ELISA) for PRRSV antibodies using 1) PRRSV antibody-negative reference sera (n=30), 2) naturally infected pig sera (n=40), 3) sequential sera obtained from 24 experimentally infected pigs, and 4) sera submitted to three state diagnostic laboratories (n=200). The recombinant antigen assay yielded an average increased sensitivity of 10% over the commercial PRRSV ELISA. The negative controls (group 1 sera) showed no difference between the two assays. This comparison confirmed that the recombinant antigen-specific assay was more sensitive than the commercial ELISA and is well suited for routine confirmation of the presence of PRRSV antibodies. |