Submitted to: Journal of Microbiological Methods
Publication Type: Abstract Only
Publication Acceptance Date: 2/2/2000
Publication Date: N/A
Technical Abstract: While a commercially available system for microbial identification by fatty acid analysis (MIDI) is available, it requires approximately 40 mg of biomass. Various authors have published methods for fatty acid analysis of single colonies, but no database of organisms has been developed for those methods. A modification of the MIDI system to increase sensitivity while maintaining relative retention times and peak areas allows the standard peak naming tables and libraries of organisms to be used with single colonies.