Submitted to: NE112 Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 11/15/1999
Publication Date: N/A
Technical Abstract: This is the IDRL annual report submitted to CSREES Regional Project NE-112 Resistance to Mastitis in Dairy Cattle (Drs. Guidry and Paape). Briefly: 1) Cell surface factors responsible for movement of white blood cells into the mammary gland decrease shortly after paturition and could be responsible for the increase in infection rate during this period: 2) Peroxynitrite produced during acute mastitis could be responsible for the observed tissue damage; 3) Identified a serotype of Staphylococcus aureus that accounts for the 60% of S. aureus not accounted for in vaccines currently used; 4) A S. aureus surface protein that causes S. aureus to adhere and penetrate mammary cells was identified and will be used in a vaccine to prevent infection by this organism; 5) A single injection of proteins, incorporated into biodegradable microspheres and injected into mice and cattle produced a highly significant sustained immune response that lasted for 52 weeks. This delivery system could be used to administe vaccines for the prevention of mastitis; and 6) Antibodies that recognize bacteria and antibodies that recognize white blood cells were linked as "bispecific antibodies" and will be used to improve the recognition of infectious organisms white blood cells thus enhancing the animal's defense against infection.