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Title: ANIONIC PEPTIDE PRODUCTION IN SELECTED EXTRA-PULMONARY BOVINE TISSUES

Author
item FALES-WILLIAMS, A - IOWA STATE UNIV.,AMES,IA.
item Brogden, Kim
item HUFFMAN, E - IOWA STATE UNIV.,AMES,IA.
item ACKERMANN, M - IOWA STATE UNIV.,AMES,IA.

Submitted to: American College of Veterinary Pathologists Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 7/14/1998
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: The absence or inactivation of epithelial antimicrobial peptides (AMP) may lead to increased susceptibility to microbial colonization of mucosal barriers. Anionic peptide (AP) is a recently described AMP first detected in ovine bronchoalveolar lavage fluid, and later determined to be present in ovine, bovine and human lung tissue. AP is a small peptide composed of seven aspartic acid residues which result in an anionic charge. AP requires zinc as a co-factor for microbial killing. AP immunoreactivity (AP-IR) has been documented in the lung of cattle, but its presence in other tissues has yet to be determined. To assess presence of AP outside of the lower respiratory tract, selected tissues were collected for immunohistochemistry (IHC) and western blots from four healthy adult cows and one fetus. Within the upper respiratory tract (nasal turbinate and trachea), nuclear AP-IR is present in surface epithelium and serous submucosal glands, while mucus-producing glands lack AP-IR. In addition, tracheal epithelium has strong cytoplasmic AP-IR. Basal and mid-level epithelial cells of the skin and tongue show moderate nuclear AP-IR. Abomasal parietal cell cytoplasm and nuclei are moderately positive, while chief cells are weakly positive. Renal epithelium had strong nuclear AP-IR clei of virtually all cell types of adrenal gland have strong AP-IR. Nuclei of rumen epithelium have strong AP-IR. Nuclei of hepatocytes are weakly positive. By western blot analysis, multiple bands were seen and ranged from 6.5 kD to 66.0 kD, with the most common band at 31 kD). In conclusion, AP-IR was seen in both the nucleus and cytoplasm of upper respiratory tract and oral cavity tissues and in the nucleus of other select tissues. Whether nuclear staining, void of cytoplasmic staining, is