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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Grain Quality and Structure Research » Research » Research Project #442945

Research Project: Evaluate and Compare Current Amylose Quantification Methods for Grain Sorghum

Location: Grain Quality and Structure Research

Project Number: 3020-43440-002-028-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Sep 1, 2022
End Date: Aug 31, 2025

Develop a quick and reliable method to measure amylose contents in sorghum grain and understand how the results from the method are compared with those from other methods.

1. Identify and collect sorghum varieties and breeding lines for the project. 2. Develop a quick and easy screen method to detect contamination of normal sorghum grains in waxy grain. 3. Evaluate and compare different methods (potentiometric, colorimetric, concanavalin A, GPC/SEC, and DSC) for determining amylose contents in sorghum grain/flour. 4. Evaluate GxE effects on amylose contents in grain sorghum and their functionality in food applications.