Location: Grain Quality and Structure Research
Project Number: 3020-43440-002-008-S
Project Type: Non-Assistance Cooperative Agreement
Start Date: Sep 25, 2017
End Date: Sep 24, 2022
Objective:
The objective of this mouse study is to investigate whether feeding high phenolic sorghum bran and high tannin sorghum bran influence development of polyps and histological changes in mouse colon tissues.
Approach:
ApcMin+ mice with truncated deletion of tumor suppressor (Apc) can be used as a mouse model for human familial adenomatous polyposis (FAP) syndrome. The treatment of 2% DSS (w/v) administered to ApcMin+ mouse led to the development of multiple colon tumors, which are histopathologically adenoma and adenocarcinoma.
Twenty ApcMin+ mice (C57BL/6J-ApcMin/J, male) will be purchased from the Jackson laboratories (Bar Harbor, ME) and assigned to one of following treatment groups; 1) control (n=5), 2) cellulose (n=5), 3) high phenolic sorghum bran (n=5) and 4) high tannin sorghum bran (n=5). Different diets will be fed to mice at 4 weeks of age. At 5 weeks of age, all mice will be given with 2% DSS (w/v) in drinking water for 1 week and maintained with same different diets for additional 4 weeks. Body weight will be measured weekly and any potential toxicity will be monitored. At 9 weeks of age, the mice will be euthanized by overdose of CO2. The entire intestinal tract will be removed, flushed with cold saline, opened longitudinally, and fixed flat in saline. The number, size, and location of polyps will be determined from proximal and distal parts of colon tissues. Both normal and neoplastic tumors will be used for immunohistochemistry and immunoblot. All of the experiments will be conducted after IACUC approval and under the guidance of the UMD IACUC.
